Journal: PLoS ONE

Article Title: MMP-9 and CXCL8/IL-8 Are Potential Therapeutic Targets in Epidermolysis Bullosa Simplex

doi: 10.1371/journal.pone.0070123

Figure Lengend Snippet: A. SQRT-PCR shows increased matrix metalloproteinase mRNA expression in EBS-DM cell lines (n = 4). B. MMP-9 ELISA of 48-h-conditioned cell culture supernatant shows 2-fold upregulation of MMP-9 in KEB-7 and 46-fold upregulation in EBDM-1 at the protein level (n = 4). C. MMP-9 levels were highly increased in EBS patients blister fluids compared to healthy controls (n = 3 to n = 4). The numbers correlate with . Student`s t -test was performed with p values: * ≤0.05, ** ≤0.01, *** ≤0.005, ΔΔ≤0.0005, ΔΔΔ≤0.0001. (In 2C, the Student’s t -test compared the entire patient group to the entire control group).

Article Snippet: Protein levels of KLK5, MMP7, MMP9, CXCL1, CXCL8/IL-8, CXCL11 and CXCL14 were determined in 48-h-conditioned cell culture supernatant and in patients blister fluids by using Quantikine® ELISA (Human KLK5, # DKK500, R&D Systems; Human MMP7, # DMP700, R&D Systems; Human MMP9, # DMP900, R&D Systems; Human CXCL1/GROα, # DGR00, R&D Systems; Human CXCL8/IL-8, # D8000C, R&D Systems; Human CXCL11/I-TAC, # DCX110, Human CXCL14/BRAK, # DY866, R&D Systems) following the manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Control

Journal: PLoS ONE

Article Title: MMP-9 and CXCL8/IL-8 Are Potential Therapeutic Targets in Epidermolysis Bullosa Simplex

doi: 10.1371/journal.pone.0070123

Figure Lengend Snippet: A. SQRT-PCR of chemokine mRNA expression in NEB-1, KEB-7 and EBDM-1 (n = 3 to n = 5). CXCL1 , CXCL8/IL-8 and CXCL14 expression was increased in KEB-7. Only CXCL11 and CXCL14 were increased in EBDM-1. B. CXCL8/IL-8 ELISA of 48-h-conditioned cell culture supernatant showed 2-fold upregulation at the protein level in KEB-7 but not in EBDM-1, which correlates with the SQRT-PCR results (n = 4). C. CXCL8/IL-8 concentrations were highly increased in EBS patients blister fluids. In blister fluids of healthy controls no CXCL8/IL-8 was detectable (n = 3 to n = 4). The numbers correlate with . Student’s t -test was performed with p values: * ≤0.05, *** ≤0.005, Δ≤0.001, ‡ = no significant difference between investigated cell lines. (In 6C, the Student’s t -test compared the entire patient group to the entire control group).

Article Snippet: Protein levels of KLK5, MMP7, MMP9, CXCL1, CXCL8/IL-8, CXCL11 and CXCL14 were determined in 48-h-conditioned cell culture supernatant and in patients blister fluids by using Quantikine® ELISA (Human KLK5, # DKK500, R&D Systems; Human MMP7, # DMP700, R&D Systems; Human MMP9, # DMP900, R&D Systems; Human CXCL1/GROα, # DGR00, R&D Systems; Human CXCL8/IL-8, # D8000C, R&D Systems; Human CXCL11/I-TAC, # DCX110, Human CXCL14/BRAK, # DY866, R&D Systems) following the manufacturer’s protocol.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Control

Journal: bioRxiv

Article Title: Identification of CD317-Positive Pro-inflammatory Immune Stromal Cells in Human Mesenchymal Stromal Cell Preparations

doi: 10.1101/2022.02.10.479972

Figure Lengend Snippet: (A) Comparative mRNA expression of ICAM-1 in MSC lines and primary cells sorted by CD317 expression (RNA was extracted from 3 different donors or 5 cell line passages; qPCR performed in triplicate, mean shown ± SEM). (B) Mean fluorescence intensity of ICAM-1 expression on the cell surface of MSC lines and primary MSCs differentially gated by CD317 staining (MSCs from 5 different donors or 4 different passages of MSC lines were stained for flow cytometry, mean shown ± SEM). (C)/(D) Comparative (mean ± SEM) mRNA expression of CXCL10 (red) and CXCL11 (blue) in MSC lines/ primary MSCs sorted for CD317 expression (RNA was extracted from 7 different donors/7 different cell passages; experiments were performed in triplicate). (E/F) CXCL10 secretion by MSC lines prior to IFN-γ priming and after priming with baseline (unprimed) secretion subtracted (mean ± SEM, n=2). (G/H) Comparative mRNA expression of 8 IFN-γ signature genes in MSC lines/primary MSCs sorted by CD317 expression (RNA was extracted from 5 different donors/5 different cell passages; experiments were performed in triplicate, mean shown ± SEM). (I)/(J) IFN-γ score for MSC lines/primary MSCs sorted by CD317 expression (n=5)*/** = significance at P<0.05/0.01 using an appropriate statistical test.

Article Snippet: To detect secreted proteins, supernatants from 100,000 cells incubated in 2.5 ml of serum free DMEM for 24 hours was analysed for secreted proteins by enzyme-linked immunosorbent assays (ELISA) using ELISA kits for CXCL10, CXCL11 (BioLegend); CCL2 (eBioscience); and SAA4 (Stratech) following manufacturers instructions.

Techniques: Expressing, Fluorescence, Staining, Flow Cytometry

Journal: Indian Journal of Dermatology

Article Title: The Effect of Transplantation of Cultured Autologous Melanocytes on CXCL9, CXCL10 and CXCL11 Expressions in Vitiligo

doi: 10.4103/ijd.ijd_925_22

Figure Lengend Snippet: Expression levels of serum CXCL9, CXCL10 and CXCL11 in different types of vitiligo patients

Article Snippet: Before the intervention, 5 ml of peripheral venous blood (PBMC) was collected from all the participants to detect the concentrations of CXCL9, CXCL10 and CXCL11 by ELISA kit (Wuhan, Boster Biological Technology, China).

Techniques: Expressing

Journal: Indian Journal of Dermatology

Article Title: The Effect of Transplantation of Cultured Autologous Melanocytes on CXCL9, CXCL10 and CXCL11 Expressions in Vitiligo

doi: 10.4103/ijd.ijd_925_22

Figure Lengend Snippet: Expression levels of serum CXCL9, CXCL10 and CXCL11 in vitiligo patients and healthy controls

Article Snippet: Before the intervention, 5 ml of peripheral venous blood (PBMC) was collected from all the participants to detect the concentrations of CXCL9, CXCL10 and CXCL11 by ELISA kit (Wuhan, Boster Biological Technology, China).

Techniques: Expressing

Journal: Indian Journal of Dermatology

Article Title: The Effect of Transplantation of Cultured Autologous Melanocytes on CXCL9, CXCL10 and CXCL11 Expressions in Vitiligo

doi: 10.4103/ijd.ijd_925_22

Figure Lengend Snippet: Expressions levels of serum CXCL9, CXCL10 and CXCL11 in vitiligo patients of different stages

Article Snippet: Before the intervention, 5 ml of peripheral venous blood (PBMC) was collected from all the participants to detect the concentrations of CXCL9, CXCL10 and CXCL11 by ELISA kit (Wuhan, Boster Biological Technology, China).

Techniques:

Journal: Indian Journal of Dermatology

Article Title: The Effect of Transplantation of Cultured Autologous Melanocytes on CXCL9, CXCL10 and CXCL11 Expressions in Vitiligo

doi: 10.4103/ijd.ijd_925_22

Figure Lengend Snippet: Expression levels of serum CXCL9, CXCL10 and CXCL11 in stable vitiligo before and after the operation

Article Snippet: Before the intervention, 5 ml of peripheral venous blood (PBMC) was collected from all the participants to detect the concentrations of CXCL9, CXCL10 and CXCL11 by ELISA kit (Wuhan, Boster Biological Technology, China).

Techniques: Expressing